My Scientific Papers - From thesis of my M. Sc. 

(1)Effects of ascorbic acid on in vitro cultivation of H. diesingi eggs.

Authors

Gosavi, A. V.; Renapurkar, D. M.; Kulkarni, A. B.

Journal-Bulletin of Haffkine Institute 1980 Vol. 8 No. 1 pp. 15-20

Record Number-19840816107

Abstract

Eggs dissected from the ovaries of Hammerschmidtiella diesingi were incubated in Locke's solution containing 0.1% (w/v) DL-ascorbic acid. The presence of ascorbic acid enhanced embryonic development, significantly so between 8 and 42 h of incubation. Thereafter the cultures were similar to those which lacked ascorbic acid. At 36 h, 60% of the eggs incubated with ascorbic acid were at the active embryonic stage, whereas in control cultures at this time only 10% were at this stage and 60% were at the pre-active embryonic stage.

 

(2) Oxygen uptake studies on hammerschmidtiella diesingi.

Gosavi, A. V.; Renapurkar, D. M.; Kulkarni, A. B., 1978-Bulletin of Haffkine Institute 6(3): 73-78

 

In chemotherapeutic treatment of helminthiasis, sensitivity of parasite to anthelmintic drugs depends to a large extent on parasite's metabolic pathways, which may be primarily either aerobic or anaerobic. But it has been difficult to understand the ability of some of the worms to derive energy aerobically and anaerobically. Anthelmintic drugs affect particularly carbohydrate metabolism which holds the key role as the source of energy in helminth parasites. Studies on carbohydrate metabolism of helminths have shown 2 main metabolic pathways. One pathway shows predominance of glycolytic, as the entire source of energy; in the other, the glycolytic pathway is shuttled at phosphoenol pyruvate stage to oxaloacetate and malate through CO2 fixation. Some biochemical features of carbohydrate metabolism 11 parasites were studied. A simple invertebrate host-parasite model of Periplaneta americana and its nematode parasite, H. diesingi was developed for the preliminary studies. The O2 uptake study on H. diesingi was carried out by using Warburg manometric techniques. Intact worms and homogenates were incubated for 3-8 h in glucose, pyruvate and acetate substrates. The O2 uptake was equivalent with these substrates. In sugars, more in galactose than glucose and mannose was found. The significance of these results were discussed.

(3) Effect of coconut milk on in vitro embryonic development of Hammerschmidtiella diesingi, Leidy Nematoda: Thelastomatidae

Gosavi, AV.; Renapurkar, DM.; Kulkarni, AB., 1981: Effect of coconut milk on in vitro embryonic development of Hammerschmidtiella diesingi, Leidy Nematoda: Thelastomatidae. Biovigyanam, 72: 187-189

 

(4) Infection patterns in multiple species of nematodes parasitic in Periplaneta americana L

Renapurkar, DM.; Gosavi, AV.; Arekar, AS., 1994: Infection patterns in multiple species of nematodes parasitic in Periplaneta americana L. Indian Journal of Helminthology. 1993( ); 451-2: 54-63.

                                                              **************